Figure3. Use in IHC-P,DAB staining
WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA
Catalog
A0P20620A 1mg
活性：
Human, Mouse, Rat, Yeast, Plant, Zebrafish, Bovine and more
应用：
WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA
结合物：
Conjugate Unconjugated
论文：
1426
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
联系电话：
背景介绍
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
产品信息
10782-2-AP targets TDP-43 in WB, RIP, IP, IHC, IF, IEM, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
| Tested Applications | FC, IF, IHC, IP, WB, ELISA |
| Cited Applications | ChIP, CoIP, ELISA, IEM, IF, IHC, IP, RIP, WB |
| Tested Reactivity | Human, Mouse, Rat, Zebrafish |
| Cited Reactivity | Human, Mouse, Rat, Chicken, Horse, Yeast, Seal, Monkey, Zebrafish, Hamster |
| Immunogen | fusion protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | TAR DNA binding protein |
| Synonyms | ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 44 kDa |
| GenBank accession number | BC001487 |
| Gene symbol | TARDBP |
| Uniprot | Q13148 |
| Gene ID (NCBI) | 23435 |
| RRID | AB_615042 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
使用和存储
使用和储存
经过测试的应用
经过测试的应用
实验方案
| PRODUCT SPECIFIC PROTOCOLS | |
| WB protocol for TDP-43 antibody 10782-2-AP | Download protocol |
| IHC protocol for TDP-43 antibody 10782-2-AP | Download protocol |
| IF protocol for TDP-43 antibody 10782-2-AP | Download protocol |
| IP protocol for TDP-43 antibody 10782-2-AP | Download protocol |
| FC protocol for TDP-43 antibody 10782-2-AP | Download protocol |
| STANDARD PROTOCOLS | |
| Click here to view our Standard Protocols | |
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