GAPDH Polyclonal antibody-模型资源库_基因_影像分析平台

GAPDH Polyclonal antibody

Cat No

R20000Hu00

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12

Uniprot

P04406

Full name

glyceraldehyde-3-phosphate dehydrogenase

概述

质检展示

WB analysis using 60004-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

IF Staining of HeLa using 60004-1-Ig

Immunofluorescent analysis of EA treated HeLa cells using 60004-1-Ig(GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

IF Staining of HeLa using 60004-1-Ig

IF Staining of HeLa using 60004-1-IgIF Staining of HeLa using 60004-1-Ig

质检说明

WB=1:5000-40000 ELISA=1:5000-10000 IHC-P=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

文献引用

Yu, ShuJun. et al. Low temperature plasma protects against inflammatory agents-mediated dysfunction of theca cells via enhancing MANF expression. MOL BIOL REP. 2023 Jan;:1-13 WB ; Mouse.

产品概述

Host: Rabbit

Reactivity: Human, Mouse, Rat

Class: Polyclonal

Immunogen: P25369Hu00

Application: WB=1:5000-40000 ELISA=1:5000-10000 IHC-P=1:100-500

Purification Method: Protein A/G and antigen-specific affinity chromatography

Conjugate: Unconjugated

Endotoxin: Conjugate<1.0 EU per ug of the protein as determined by the LAL method.

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, thereversible oxidative phosphorylation of glyceraldehyde-3-phosphatein the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Calculated molecular weight: 37kDa

Observed molecular weight: 37kDa

Form: Liquid

Storage Buffer: PBS with Proclin300 and 50% glycerol, pH 7.3.

Storage Conditions: Storage Conditions

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